Dal Alert!

Receive alerts from Dalhousie by text message.

X

Vasantha Rupasinghe

Professor and Killam Chair in Functional Foods and Nutraceuticals

Department of Environmental Sciences

Email: vrupasinghe@dal.ca
Phone: 902.893.6623
Mailing Address: 
Faculty of Agriculture, Dalhousie University
Rm 219-C Cox Institute Building
50 Pictou Rd. PO Box 550
Truro, NS, B2N 5E3
 
Research Topics:
  • Functional foods and nutraceuticals
  • Plant-based antioxidants and their health benefits
  • Flavonoids (polyphenols) and their disease prevention potential and analysis using mass spectrometry
  • Medicinal biochemistry: human cell based assays and experimental animal models
  • Postharvest biology, novel and improved value-added processing technologies of cool climate fruits and medicinal plants

Teaching

Undergraduate

  • CHMA 3001 Biochemistry (Formerly Biochemical Pathways)
  • FOOD 3001 Functional Foods and Nutraceuticals
  • FOOD 4000 Directed Studies in Food & Bioproduct Science

Graduate

  • AGRI 5730 Directed Studies in Food & Bioproduct Science
  • AGRI 5710 Graduate module: Plant Secondary Metabolites
  • AGRI 5710 Graduate module: Biochemistry of Fruit Ripening
  • AGRI 5710 Mass Spectrometry: Fundamentals and Applications (with Dr. J. Hoyle)

Research Interests

Medicinal biochemistry of phytochemicals; Disease-fighting properties of plant flavonoids and their analogues; Polyphenol micronutrients of cool climate fruits and disease prevention.

Development and evaluation of value-added health food and feed ingredients, natural food additives, functional foods, natural health products and other horticultural bio-products.

Postharvest biology and technology of fruits; Biochemistry of fruit ripening; Novel and improved value-added processing technologies; Shelf life and quality.

Research Program

Flavonoids in Plant Food and their Health Benefits

In our research, we strongly believe what Hippocrates (460-377 B.C.), the father of medicine, stated thousands of years before: "Let thy food be thy medicine and thy medicine be thy food”.  Rapid advancements in food production after the green revolution and changing life style has made our regular diet nutritionally imbalanced. Now, not only the industrialized countries but rapidly developing countries of the world face significant challenges from epidemics of chronic disease that are directly or indirectly linked to diet, lifestyle and the environment. For example, deaths from cancer worldwide are projected to continue rising, World Health Organization has estimated 13.1 million deaths in 2030 due to cancer. Cancer treatment in many countries has already become a social-economic challenge due to the treatment and patient-care expenses and medical infrastructure required. We believe, prevention as the most cost-effective, long-term approach for controlling disorders such as cancer.  The last two decades of scientific evidence, epidemiological evidence and recent meta-analysis of prospective cohort studies clearly demonstrates the relationship between habitual adequate consumption of fruits and vegetables and reduce incidence of oxidative-stress and chronic inflammation regulated disorders. 

Our recent research were mainly focused on flavonoids, the major antioxidant components in fruits, which have remarkable power in preventing the development of chronic diseases and promoting human health and wellness. In particular, our research hypotheses is that some unique flavonoids in cool climate fruits such as apples and berries can play as the major biologically active components of our diet to reduce the risk of cardiovascular and neurodegenerative disorders and various cancers. My research team has been attempting to answer to the questions of: what are the most important types of dietary flavonoids? what amount of flavonoids are needed for the therapeutic efficacy? could isolated flavonoid supplements provide the efficacy and safety? what are their mechanism of action(s) at cellular level? Our applied research is designed to translate the new discoveries into functional foods and nutraceuticals. 

(A) Cardio-protective properties of fruit flavonoids and their products

Using pre-clinical studies, we have demonstrated that a specific apple flavonoid extract (AFE) possesses multiple target-based mechanisms toward the prevention and treatment of cardiovascular and coronary heart diseases. The apple flavonoids showed the reduction of the risk of atherosclerosis by remarkably decreasing the oxidation of low density lipoprotein (LDL)-cholesterol in vitro. When the diet of hypercholesterolemic male Golden Syrian hamsters was supplemented with the AFE, plasma total cholesterol and LDL-cholesterol levels were significantly reduced compared to the control. Similarly, when the extract was supplemented in the diet of hyperlipidemic male Wister rats, not only LDL-cholesterol level significantly reduced but also high density lipoprotein (HDL)-cholesterol level significantly increased. When lipopolysaccharide (LPS) was used to induce inflammation of Wister rats, pro-inflammatory markers (IL-6 and CRP) in blood were significantly reduced by the extracts indicating the anti-inflammatory properties. AFE and their constituent flavonoids inhibited the angiotensin converting enzyme (ACE), a therapeutic target of anti-hypertension drugs, at very low concentrations (20 nM to 3 µM) in HUVEC cells. The dietary supplementation of AFE and a fruit-based functional beverage reduced the blood pressure of spontaneously hypertensive rats. 

• Gunathilake, K.D.P.P., H.P.V. Rupasinghe, and Y. Wang. 2013. J. Functional Foods. 5: 1392-1401. 

• Gunathilake, K.D.P.P., H.P.V. Rupasinghe, N.L. Pitts. 2013. Food Research International. 52:535-541. 

• Thilakarathna, S.H., H.P.V. Rupasinghe, P.W. Needs. 2013. Food Chem. 138:463-470. 

• Thilakarathna, S.H., Y. Wang, H.P.V. Rupasinghe, K. Ghanam. 2012. J. Functional Foods. 4: 963-971. 

• Balasuriya, N. and H.P.V. Rupasinghe. 2012. Food Chem. 135, 2320-2325. 

• Balasuriya, N. and H.P.V. Rupasinghe. 2011. Functional Foods for Health and Disease. 5:172-188.

(B) Flavonoids-based natural health products for neurodegenerative diseases

Despite modern drug discovery approaches, no therapeutic product is available to halt the degenerative processes responsible for stroke, multiple sclerosis (MS) or Alzheimer’s disease. We have found a specific flavonoid fraction derived from apple peels (AF4) with strong neuroprotective and anti-inflammatory effects when examined in mice subjected to hypoxic-ischemic (HI) brain damage, an experimental model for stroke. Oral administration of AF4 (25 mg/kg/d) for at least three days prior to HI markedly reduced subsequent motor impairments, brain damage, and inflammation after an experimental stroke. AF4-mediated neuroprotection was accompanied by changes in gene expression suggestive of improved mitochondrial function and activation of pathways that promote cell survival. AF4 has also reduced clinical signs of MS in an experimental autoimmune encephalomyelitis (EAE) model of mice by decreasing neuroinflammatory damage in the spinal cord and improving the expression of genes necessary for remyelination. Similarly, flavonoids-rich fraction isolated from partridgeberry (Vaccinium vitis-idaea) exhibited cytoprotection ability in peroxyl radical-induced fibroblasts through activation of the transcription factor Nrf2-antioxidant responsive element (ARE) pathway and protection of rat primary cortical and hippocampal neurons against β-amyloid toxicity and oxygen-glucose deprivation-reperfusion-induced injury via suppression of inflammatory adipokines and regulation of hypoxia-inducible factor 1-alpha (HIF-1α) and peroxisome proliferator-activated receptor gamma (PPARγ).

• Bhullar, K.S. and H.P.V. Rupasinghe. 2015. Food Research International. 74: 237–249.

• Bhullar, K.B. and H.P.V. Rupasinghe. 2015. Food Chemistry. 168:595-605.

• Bhullar, K.S. and H.P.V. Rupasinghe. 2015. Nutritional Neuroscience. doi.org/10.1179/1476830515Y.0000000026.

• Warford, J., R. Quinton, D. Jones, M. Nichols, V. Sullivan, H.P.V. Rupasinghe, G.S. Robertson. 2014. Journal of Neuroinflammation 268:71-83. 

• Bhullar, K.S. and H.P.V. Rupasinghe. 2013. Oxidative Medicine & Cellular Longevity. Article ID 891748. 2013:1-18. 

• Keddy, P., K. Dunlop, J. Warford, M.L. Samson, D. Jones, H.P.V. Rupasinghe, G.S. Robertson. 2012. PLoS ONE 7:1-18. 

• Jones, Q.R.D, J. Warford, H.P.V. Rupasinghe, G.S. Robertson. 2012. Trends in Pharm. Sci. 33:602-610. 

(C) Novel anti-cancer therapeutic agents synthesized from food biomolecules:

To enhance the bioavailability and biological activities of flavonoids, we have synthesized a series of novel flavonoid derivatives with higher hydrophobicity. The lipase-catalyzed acylation of phloridzin (PZ) and isoquercetrin (Q3G) with six long chain fatty acids was optimized. We have identified an effective and safe flavonoid derivative with potential for treating hepatocellular carcinoma, acute monocytic leukemia, and breast cancer. The antiproliferative potency of fatty acid esters of PZ was comparable to the potency of the chemotherapeutic drugs. The fatty acid esters of PZ inhibited DNA topoisomerases IIα activity that might induce G0/G1 phase arrest, induced apoptosis via activation of caspase-3, and decreased ATP level and mitochondrial membrane potential in HepG2 cells. Antiproliferative efficacy of one of the derivatives, docosahexaenoic acid acylated PZ (PZ-DHA) was investigated using a triple negative breast cancer cell line (MDA-MB-231). PZ-DHA, but not PZ, caused the death of MDA-MB-231 in vitro which was both time- and dose-dependent. PZ-DHA-induced apoptosis was indicated by DNA fragmentation and activation of caspase 3/7. PZ-DHA showed low cytotoxicity toward human normal cells. Anticancer efficacy observed in cell cultures and mice suggest PZ-DHA as a potential treatment for breast cancer. Advanced pre-clinical experiments of intravenous injection and oral administration of PZ-DHA using animal models are in progress. 

• Nair, S.V.G., Ziaullah, and H.P.V. Rupasinghe. 2014. PLoS ONE 9(9): e107149. 

• Rupasinghe, H.P.V. 2015. Acylated derivatives of phloridzin and isoquercetrin as anticancer therapeutics and methods of use thereof. WO2015/019193A2. (Patent-pending).

• Ziaullah and H.P.V. Rupasinghe. 2013. Tetrahedron Letters 54:1933-1937. 

• Ziaullah, K.S. Bhullar, S.N. Warnakulasuriya, and H.P.V. Rupasinghe. 2013. Bioorg. Med. Chem. 21:684-692. 

• Thilakarathna, S. and H.P.V. Rupasinghe. 2013. Nutrients 5:3367-3387. 

(D) Prevention of omega-3 lipid oxidation by apple peel flavonoids

Natural food antioxidants for stabilization of omega-3 polyunsaturated fatty acid (PUFA) have received much attention due to the restrictions of synthetic antioxidants in foods in some countries. The effectiveness of quercetin glycosides (QG) in inhibiting lipid oxidation in oil-in-water emulsion systems when oxidation was induced by heat, light, peroxyl radical or ferrous ion was greater than that of α-tocopherol. Antioxidant activity of QG was greater than butylated hydroxytoluene in bulk fish oil. QG-rich ethanol extracts of apple peels strongly inhibited omega-3 PUFA oxidation. QG isolated from apple peels showed ability to stabilize omega-3 PUFA-oxidation induced heat.  

• Sekhon, S., H.P.V. Rupasinghe, and F. Shahidi. 2013. Food Chem. 140:189-196.

• Huber, G.M., H.P.V. Rupasinghe, and F. Shahidi. 2009. Food Chem. 117: 290-295.

• Huber, G.M., and H.P.V. Rupasinghe. 2009. J. Food Sci. 74:693-700.

• Rupasinghe, H.P.V., N. Erkan, and A. Yasmin. 2010. J. Agric. Food Chem. 58:1233-1239.

• Rupasinghe, H.P.V. and A. Yasmin. 2010. Molecules 15:251-257.

 

Publications can be found at Research Gate

 

Memberships

• Cross Appointment, Department of Pathology, faculty of Medicine, Dalhousie University, Halifax, Nova Scotia.

• Adjunct Professor, Department of Pharmacology, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia.

• Adjunct Professor, Department Process Engineering & Applied Sciences, faculty of Engineering, Dalhousie University, Halifax, Nova Scotia.

• Adjunct Professor, Department of Food Science and Nutrition, Addis Ababa University, Addis Ababa, Ethiopia.

• Adjunct Professor, Postgraduate Faculty, Sichuan Agricultural University, China.

• Board of Director, Canadian Institute of Food Science and Technology (CIFST).

• Chair, Scientific Expert Council, CIFST

Memberships continued

Publications

Rupasinghe, H.P.V., S. Sekhon-Loodu, T. Mantso, M.I. Panayiotidis. 2016. Phytochemicals in regulating fatty acid β-oxidation: Potential underlying mechanisms and their involvement in obesity and weight loss. Pharmacology & Therapeutics. (In Press).

• Warnakulasuriya, S.N., Ziaullah, and H.P.V. Rupasinghe. 2016. Novel long chain fatty acid derivatives of quercetin-3-O-glucoside reduce cytotoxicity induced by cigarette smoke toxicants in human fetal lung fibroblasts. European Journal of Pharmacology. 781:128-138.

• Warnakulasuriya, S.N., Ziaullah, and H.P.V. Rupasinghe. 2016. Long chain fatty acid esters of quercetin-3-O-glucoside attenuate H2O2-induced acute cytotoxicity in human lung fibroblasts and primary hepatocytes. Molecules. 21:452-471.

• Jiang, Y., L. Zhang, H.P.V. Rupasinghe. 2016. The anticancer properties of phytochemical extracts from Salvia plants. Botanics: Targets and Theraphy. 6:25-44.

• Abachi, S., S. Lee and H.P.V. Rupasinghe. 2016. Molecular mechanisms of inhibition of streptococcus species by phytochemicals. Molecules. 21:215-246.

• Ziaullah and H.P.V. Rupasinghe. 2016. Sonochemical enzyme-catalyzed regioselective acylation of flavonoid glycosides. Bioorganic Chemistry. 65:17-25.

• Duffield, K.M., M.R. Ness, K.S. Bhullar, H.P.V. Rupasinghe, J. Balzarini, A. Jha. 2015.  Curcumin-inspired cytotoxic 3,5-bis(arylmethylene)-1-(N-arylmaleamoyl)-4-piperidones: A novel group of topoisomerase II alpha inhibitors. Bioorganic & Medicinal Chemistry. 23:6404-6417.

• Bhullar, K.S. and H.P.V. Rupasinghe. 2015. Partridgeberry polyphenols protect primary cortical and hippocampal neurons against β-amyloid toxicity. Food Research International. 74: 237–249.

• Arumuggam, N., N.A. Bhowmick, H.P.V. Rupasinghe. 2015. Phytochemicals targeting JAK/STAT signaling and IDO expression in cancer. Phytotheraphy Research. 29:805-817.

• Sekhon-Loodu, S. Ziaullah, and H.P.V. Rupasinghe. 2015. Docosahexaenoic acid ester of phlorizin inhibit lipopolysaccharide-induced inflammation in THP-1 differentiated macrophages. International Immunopharmacology. 25:199-206.

• Caseya, S.C., A. Amedei, K. Aquilano, A.S. Azmi, F. Benencia, D. Bhakta, A.E. Bilsland, C.S. Boosani, S. Chen, M.R. Ciriolo, S. Crawford, H. Fujii, A.G. Georgakilas, G. Guha, D. Halicka, W.G. Helferich, P. Heneberg, K. Honoki, W.N. Keith, S.P. Kerkar, S.I. Mohammed, E. Niccolai, S. Nowsheen, H.P.V. Rupasinghe, A. Samadi, N. Singh, W.H. Talib, V. Venkateswaran, R.L. Whelan, and X. Yang. 2015. Cancer prevention and therapy through the modulation of the tumor microenvironment – A review. Seminars in Cancer Biology. 35:S199-S223.

• Bhullar, K.B., A. Jha, and H.P.V. Rupasinghe. 2015. Novel carbocyclic curcumin analog CUR3d modulates genes involved in multiple apoptosis pathways in human hepatocellular carcinoma cells. Chemico-Biological Interactions. 242:107-122.

• Potter, E., M. Jha, K. Bhullar, H.P.V. Rupasinghe, J. Balzarini, and A. Jha. 2015. Investigation of fatty acid conjugates of 3,5-bisarylmethylene-4-piperidone derivatives as antitumor agents and human topoisomerase-IIα inhibitors. Journal of Bioorganic Chemistry. 23:411-421.

• Bhullar, K.B. and H.P.V. Rupasinghe. 2015. Antioxidant and cytoprotective properties of partridgeberry polyphenols. Food Chemistry. 168:595-605.

• Sekhon-Loodu, S., Ziaullah, H.P.V. Rupasinghe, Y. Wang, M. Kulka, and F. Shahidi. 2015. Novel quercetin-3-O-glucoside eicosapentaenoic acid ester ameliorates inflammation and hyperlipidemia. Inflammopharmacology. 23:173-185.

 

More publications

 

Book Chapters

Rupasinghe, H.P.V. and S. Thilakarathna. 2016. Apple juice. In: Handbook of Functional Beverages and Human Health. (Ed.) F. Shahidi and C. Alasalvar. CRC Press, Taylor & Francis Group, Boca Raton, FL, USA. 978-1-4665-9642-9. pp. 93-106.

• Ziaullah and H.P.V. Rupasinghe. 2015. Application of NMR spectroscopy in plant phenolics associated with human health. In: Application of NMR Spectroscopy in Food Sciences (Eds.) Atta-ur-Rahman and M. Iqbal Chaudhary. Bentham Science Publishers, Oak Park, IL, USA. ISBN: 978-1-60805-999-7. pp. 03-92.

• Parmar, I., K.S. Bhullar, and H.P.V. Rupasinghe. 2015. Anti-diabetic effect of ferulic acid and derivatives: An update. In: Ferulic Acid: Antioxidant Properties, Uses and Potential Health Benefits (Ed.). Bryce Warren. Nova Science Publishers, Inc. Hauppauge, NY, USA. ISBN: 978-1-63463-299-7. 

Rupasinghe, H.P.V., S. Nair, and R. Robinson. 2014. Chemopreventive properties of fruit phenolics and their possible mode of actions. In: Studies in Natural Products Chemistry. Volume 42 (Ed.) Atta-Ur-Rahman. Elsevier Science Publishers, Amsterdam. ISBN: 978-0-444-63281-4. pp. 229-266.

• Parmar, I. and H.P.V. Rupasinghe. 2014. Proanthocyanidins in cranberry and grape seeds: metabolism, bioavailability and biological activity. In: Nutraceuticals and Functional Foods: Natural Remedy (Ed.) S.K. Brar, S. Kaur and G.S. Dhillon, Nova Science Publishers. ISBN: 978-1-62948-793-9. pp. 119-145.

• Fernando, F. and H.P.V. Rupasinghe. 2013. Anticancer properties of phytochemicals present in medicinal plants of North America. In: Using Old Solutions to New Problems - Natural Drug Discovery in the 21st Century. (Ed.) Marianna Kulka. InTech - Open Access Publisher. Rijeka, Croatia. ISBN:  978-953-51-1158-0.  pp. 159-180.

Rupasinghe, H.P.V. and A.P.K. Joshi. 2013. Phytochemicals and health benefits of dried apple snacks. In: Dried Fruits: Phytochemicals and Health Effects (Ed.) C. Alasalvar and F. Shahidi, Wiley-Blackwell Publishers, Inc. Oxford, UK. ISBN: 978-0-8138-1173-4. pp 213-225.

Rupasinghe, H.P.V. and L. J. Yu. 2013. Value-added fruit processing for human health. In: Food Industry (Ed. I. Muzzalupo, InTech - Open Access Publisher. Rijeka, Croatia.. ISBN: 978-953-51-0911-2. pp. 145-162

• Warnakulasooriya, S.N. and H.P.V. Rupasinghe. 2012. Physiological role of eicosapentaenoic acid (EPA) in reducing the coronary heart diseases. In: Eicosapentaenoic Acid: Sources, Health Effects and Role in Disease Prevention. Nova Science Publishers, Inc. Hauppauge, NY, USA. ISBN: 978-1-62257-480-3. pp. 143-154.

Rupasinghe, H.P.V., S. Thilakarathna, and S. Nair. 2012. Polyphenols of apples and their potential health benefits. In: Polyphenols: Chemistry, Dietary Sources and Health Benefits (Eds.) J. Sun, K. N. Prasad, A. Ismail, B. Yang, X. You and L. Li, Nova Science Publishers, Inc. Hauppauge, NY, USA. ISBN: 978-1-62081-868-8. pp. 333-368.

Rupasinghe, H.P.V. and L. J. Yu. 2012. Emerging preservation methods for fruit juices and beverages. In: Food Additives (Ed. Y. El-Samragy), InTech - Open Access Publisher. Rijeka, Croatia. ISBN: 979-953-307-112-0. pp. 65-82.

• Kathirvel, P. and H.P.V. Rupasinghe. 2012. Plant-derived antioxidants as potential omega-3 PUFA stabilizers. In: Fish Oil: Production, Consumption and Health Benefits (Ed.) M. Van Dijk and J. Vitek, Nova Science Publishers, Inc. Hauppauge, NY, USA. ISBN: 978-1-61470-963-3. pp. 157-186.

Rupasinghe, H.P.V. 2008. The role of polyphenols in quality, postharvest handling, and processing of fruits. In: Postharvest Biology and Technology of Fruits, Vegetables and Flowers (Ed.) Paliyath, G., Murr, D.P., Handa, A.K., and S. Lurie, S. Blackwell Publishing. ISBN: 978-0-8138-0408-8. pp. 260-281.

• Schofield, A, H.P.V. Rupasinghe, and G. Paliyath. 2008. Biosynthesis of isoprenoid in fruits and their impact on fruit quality. In: Postharvest Biology and Technology of Fruits, Vegetables and Flowers (Ed.) Paliyath, G., Murr, D.P., Handa, A.K., and S. Lurie, S. Blackwell Publishing. ISBN: 978-0-8138-0408-8. pp. 282-300.

 

Conference Proceedings

• Amararathna, D.I.M., D. Chung, G. Dellaire, D.W. Hoskin M. Johnston and H.P.V. Rupasinghe. 2016. Polyphenols-rich fruit extracts prevent tobacco specific nitrosamine-induced DNA damage in lung epithelial cells. 17th IASLC World Conference on Lung Cancer, Vienna, Austria. (December 4-7, 2016).

• d’Entremont, C., S. Macé, H.P.V. Rupasinghe, and L. Truelstrup-Hansen. 2016. Use of plant essential oils to control single and mixed species biofilms formed by foodbourne pathogens and spoilage bacteria. Food Micro 2016, Dublin, Ireland. (July 19-22, 2016).

• Fernando, W., D.W. Hoskin, H.P.V. Rupasinghe. PZ-DHA, a novel polyphenol fatty acid ester derivative suppresses breast cancer cell proliferation, metastasis and in vivo tumor growth. The 9th Annual Canadian Cancer Immunotheraphy Consortium (CCIC). Halifax, NS. (June 26-29, 2016).

• Arumuggam, N., N. Melong, C. Too, J. Berman, and H.P.V. Rupasinghe. Docosahexaenoic acid ester of phloridzin induces cytotoxic effects in human leukemic cells. The 9th Annual Canadian Cancer Immunotheraphy Consortium (CCIC). Halifax, NS. (June 26-29, 2016).

• Amararathna, D.I.M., D. Chung, G. Dellaire, D.W. Hoskin M. Johnston and H.P.V. Rupasinghe. 2016. Flavonoids-rich haskap berry extracts prevent NNK-induced DNA double strand break in lung epithelial cells. The 9th Annual Canadian Cancer Immunotheraphy Consortium (CCIC). Halifax, NS. (June 26-29, 2016).

• Wijesundara, N., R.J. Davidson and H.P.V. Rupasinghe. 2016. Inhibition of growth and biofilm formation of streptococcus pyogenes by herbal extracts. The Annual Conference of the American Society for Microbiology (ASM Microbe 2016). Boston, MA, USA (June 16-20, 2016).

• Zhang, B., N.M. Wijesundara, L. Abbey and H.P.V. Rupasinghe. 2016. Phytochemicals of Plectranthus species as potential natural anti-streptococcal agents. The 13th Annual Natural Health Product Research Society Conference, Charlottetown, PE. (May 17-20, 2016).

• Pace, E., Y. Jiang, and H.P.V. Rupasinghe. 2016. Investigation of cyanidin-3-O-glucoside isolated from haskap berries as a natural anticancer agent. The 13th Annual Natural Health Product Research Society Conference, Charlottetown, PE. (May 17-20, 2016).

• Wiser, L., M. McNeil, J. MacKeil and H.P.V. Rupasinghe. 2016. Retention of anthocyanins in haskap juice during pasteurization and storage. The 13th Annual Natural Health Product Research Society Conference, Charlottetown, PE. (May 17-20, 2016).

• Wijesundara N.M. and H.P.V. Rupasinghe. 2016. Anti-bacterial and anti-biofilm activity of selected medicinal plant extracts against Streptococcus pyogenes. The 13th Annual Natural Health Product Research Society Conference, Charlottetown, PE. (May 17-20, 2016).

• Wamboldt, S., S. Sekhon-Loodu, H.P.V. Rupasinghe. 2016. Rowanberry (Sorbus decora) extract inhibits lipid synthesis in insulin-induced adipocytes in vitro. The 13th Annual Natural Health Product Research Society Conference, Charlottetown, PE (May 17-20, 2016).

• Xu, Z. and H.P.V. Rupasinghe. 2016. Subcritical water extractions of phytochemicals. The 13th Annual Natural Health Product Research Society Conference, Charlottetown, PE (May 17-20, 2016).

• Jiang, Y., L. Zhang, and H.P.V. Rupasinghe. 2016. Anticancer properties of extracts prepared from Salvia officinalis L. and Salvia miltiorrhiza Bunge in hepatocellular carcinoma cells. The 13th Annual Natural Health Product Research Society Conference, Charlottetown, PE. (May 17-20, 2016).

• Fernando, W., M.R. Power Coombs, D.W. Hoskin, H.P.V. Rupasinghe. 2016. Sub-cytotoxic concentrations of a novel polyphenol fatty acid ester derivative arrest breast cancer cell proliferation and metastasis. Annual meeting 2016 of the American Association of Cancer Research (AACR). New Orleans, LO, USA. (April 16-20, 2016).

Rupasinghe, H.P.V. 2016. Beyond antioxidants: plant food flavonoids for human metabolic health. 2016 National Conference of the Canadian Institute of Food Science and Technology. Burnaby, BC. (February 22-23, 2016).

• Amararathna, M. and H.P.V. Rupasinghe. 2015. Dietary flavonoids in chemoprevention: A review in relation to tobacco-specific lung cancer. Canadian Cancer Research Conference 2015. Montreal, QC. (November 7-12, 2015).

• Arumuggam, N., C. Too, J. Berman, and H.P.V. Rupasinghe. 2015. Phloridzin docosahexaenoate (PZ-DHA), a novel polyphenolic derivative, shows cytotoxic effects against leukemic cells. Canadian Cancer Research Conference 2015. Montreal, QC. (November 7-12, 2015).

• Fernando, W., D.W. Hoskin, and H.P.V. Rupasinghe. 2015. PZ-DHA, a fatty acid ester of phloridzin as a selective drug candidate to treat metastatic breast cancer. Canadian Cancer Research Conference 2015. Montreal, QC. (November 7-12, 2015).

• Jiang, Y., L. Zhang, and H.P.V. Rupasinghe. 2015. Anticancer properties of polyphenols isolated from Salvia species in hepatocellular carcinoma cells. Canadian Cancer Research Conference 2015. Montreal, QC. (November 7-12, 2015).

• d’Entremont, C., S. Macé, H.P.V. Rupasinghe and L. Truelstrup-Hansen. 2015. Use of natural antimicrobial compounds for the removal of biofilms formed by Listeria monocytogenes, Staphylococcus aureus, Salmonella enterica serovar, Typhimurium and commensal bacteria. Annual Conference of the International Association for Food Protection (IAFP-2015). Portand, OR, USA. (July 25-28, 2015).

• Fernando, W., D.W. Hoskin, and H.P.V. Rupasinghe. 2014. PZ-DHA, a novel polyphenol fatty acid ester derivative, is cytotoxic for breast cancer cells. The 6th Annual Scientific Meeting of the Terry Fox Research Institute. St. John’s, NL. (May 7-9, 2015).

• Bhullar, K.S. and H.P.V. Rupasinghe. 2014. Amyloid-beta induced neurotoxicity is attenuated by partridgeberry polyphenols through Bcl-2/Bcl-xL and Keap1‐Nrf2 activation. The 21st Annual meeting of the Society for Free Radical Biology and Medicine. Seattle, WA, USA. (November 19-23, 2014).  

• Bhullar, K.S., A. Jha, H.P.V. Rupasinghe. 2014. CUR-3d: A novel curcumin inspired molecule inhibits cancer cell growth by switching on multiple apoptotic pathways. The 5th Cancer Research Conference in Atlantic Canada, Halifax, NS. (November 3-4, 2014).

• Arumuggam, N., C. Too, J. Berman, H.P.V. Rupasinghe. 2014. Cytotoxic effects of phloridzin docosahexaenoate on chronic myeloid leukemic K562 cells. The 5th Cancer Research Conference in Atlantic Canada, Halifax, NS. (November 3-4, 2014).

• Fernando, W., D.W. Hoskin, and H.P.V. Rupasinghe. 2014. Apple peel flavonoids cause the death of MDA-MB-231 cells via a reactive oxygen species-independent mechanism. The 5th Cancer Research Conference in Atlantic Canada, Halifax, NS. (November 3-4, 2014).

• Fei, C. and H.P.V. Rupasinghe. 2014. Combination effect of sorafenib and apple flavonoids AF4 on hepatocellular carcinoma HepG2 cell proliferation. The 5th Cancer Research Conference in Atlantic Canada, Halifax, NS. (November 3-4, 2014).

• Fernando, W., D.W. Hoskin, and H.P.V. Rupasinghe. 2014. Efficacy of docosahexaenoic acid acylated phloridzin: for treating triple negative breast cancer. The 18th SIS World Congress on Breast Healthcare-2014, Orlando, FL, USA. (October 16-19, 2014).

• Sekhon-Loodu, S. and H.P.V. Rupasinghe. 2014. Docosahexaenoic acid and phloridzin inhibit inflammation in lipopolysaccharide-stimulated macrophages. The 17th World Congress of Food Science and Technology and Expo (IUFoST 2014). Montreal, QC. (August 17-21, 2014). 

•  Fernando, W., D.W. Hoskin, and H.P.V. Rupasinghe. 2014. Phloretin and apple peel flavonoids cause the death of triple-negative breast cancer cells via a reactive oxygen species-independent mechanism. The 17th World Congress of Food Science and Technology and Expo (IUFoST 2014). Montreal, QC. (August 17-21, 2014). 

•  Parmar, I. and H.P.V. Rupasinghe. 2014. Characterization of phenolics in cranberry pomace with anti-oxidant, anti-tyrosinase and anti-proliferation activities in vitro. The 17th World Congress of Food Science and Technology and Expo (IUFoST 2014). Montreal, QC. (August 17-21, 2014). 

•  Parmar, I. and H.P.V. Rupasinghe. 2014. Bioactive characterization and anti-diabetic potential of polyphenol rich novel wild berry extracts from Eastern Canada. The 17th World Congress of Food Science and Technology and Expo (IUFoST 2014). Montreal, QC. (August 17-21, 2014). 

•  Bhullar, K.S. and H.P.V. Rupasinghe. 2014. Cytoprotective properties of wild partridgeberry (Vaccinium vitis-idaea L. var. minus Lodd) polyphenols in human fibroblasts. The 17th World Congress of Food Science and Technology and Expo (IUFoST 2014). Montreal, QC. (August 17-21, 2014). 

•  Boehm, M.A., I. Parmar, S. Sekhon-Loodu, and H.P.V. Rupasinghe. 2014. Anti-inflammatory properties of polyphenols of haskap (Lonicera caerulea L.) berry. The 17th World Congress of Food Science and Technology and Expo (IUFoST 2014). Montreal, QC. (August 17-21, 2014). 

•  Balasuriya, N., H.P.V. Rupasinghe, M. Sweeney-Nixson, S. McCarron, and K.  Gottschall-Pass. 2014. Diet supplemented with flavonoid-rich apple peel extract lowers blood pressure in spontaneously hypertensive rats. The 17th World Congress of Food Science and Technology and Expo (IUFoST 2014). Montreal, QC. (August 17-21, 2014). 

•  Zou, Q., S. Sekhon-Loodu, and H.P.V. Rupasinghe. 2014. Anti-inflammatory activities of selected food phytochemicals. The 17th World Congress of Food Science and Technology and Expo (IUFoST 2014). Montreal, QC. (August 17-21, 2014). 

•  Gunathilake, K.D.P.P. and H.P.V. Rupasinghe. 2014. Optimization of extraction methods and evaluation of antioxidant properties of ginger. The 17th World Congress of Food Science and Technology and Expo (IUFoST 2014). Montreal, QC. (August 17-21, 2014). 

•  Nandasiri, R. Y. Wang, and H.P.V. Rupasinghe. 2014. Blood pressure lowering and plasma lipid profile improving properties of functional fruit vinegar beverages in spontaneously hypertensive rats fed with high cholesterol diet. The 17th World Congress of Food Science and Technology and Expo (IUFoST 2014). Montreal, QC. (August 17-21, 2014). 

•  Bhullar, K.S., H.P.V. Rupasinghe, and G.S. Robertson. 2014. Evaluation of wild partridgeberry polyphenols as potential natural health product to reduce the risk of Alzheimer’s disease. The 2014 Canadian Neuroscience Conference, Montreal, QC. (May 25-28, 2014). 

•  Rupasinghe, H.P.V. 2014. Cardio- and neuro-protective functions of bioactive flavonoids present in cool climate fruits. The 5th International Conference on Natural Products for Health and Beauty. Phuket, Thailand. (May 6-8, 2014). 

•  Nair, S.V.G., Ziaullah, and H.P.V. Rupasinghe. 2014. Phloridzin fatty acid esters induce apoptosis and alter gene expression in human liver cancer cells. The Experimental Biology – 2014. San Diego, CA, USA. (April 26-30, 2014). The Journal of the Federation of American Societies for Experimental Biology. 28:S261.2 (Abstract). 

More Conference Proceedings

Selected Invited Presentations

• Plant flavonoids: Bioefficacy in chronic disease models. 2016. The 13th Annual Natural Health Product Research Society Conference, Charlottetown, PE (May 17-20, 2016).

• Natural products in food chemistry: Flavonoids and their acylated derivatives. NHPRS Pre-Conference Workshop on Analytical Technologies for Natural Product Research.  The 13th Annual Natural Health Product Research Society Conference, Charlottetown, PE (May 16, 2016).

• Disease fighting power of plant-food flavonoids. 2016. Dr. S. Paul Handa Annual Health Research Lecture – Keynote presentation. Interprofessional Health Research Day 2016. Saint John, NB. (March 18, 2016).

• The future of food: Plant food flavonoids for human health. University Seminar Series, Vancouver Island University, Nanaimo, BC. (February 24, 2016).

• Beyond antioxidants: Plant food flavonoids for human metabolic health. 2016 National Conference of the Canadian Institute of Food Science and Technology. Burnaby, BC. (February 22-23, 2016).

•    Apple Flavonoids based Functional Foods and Ingredients for the Prevention of Chronic Disorders. Euro Global Summit and Expo on Food & Beverages (Euro-Food 2015). Alicante, Spain (June 16-18, 2015).

•    Dietary flavonoids: Can they keep the doctor away? Grand Rounds Seminar Series. Department of Pathology, Faculty of Medicine, Dalhousie University, Halifax. NS. (November 13, 2014).

•    Cytoprotective and neuroprotective properties of Canadian partridgeberry. Our Food, Our Future, Research that Feeds Newfoundland and Labrador – 2014. Corner Brook, NL. (November 4-6, 2014).

•    Anti-proliferative properties of selected plant polyphenols and their analogs. Beatrice Hunter Cancer Research Institute Seminar Series. Faculty of Medicine, Dalhousie University, Halifax. NS. (June 02, 2014).

•    Cardio- and neuro-protective functions of bioactive flavonoids present in cool climate fruits. The 5th International Conference on Natural Products for Health and Beauty. Phuket, Thailand (May 6-8, 2014).

•    Haskap: Antioxidant activity and health benefits. Haskap Berry Symposium, Dalhousie University, Halifax (February 20, 2014).

•    Biological properties of fruit flavonoids and their acylated derivative long chain fatty acids. Acadia University. Wolfville, NS (October 24, 2013).

•    Biocatalytic synthesis, structural elucidation, and Biological activity of long chain fatty acid acylated derivatives of phloridzin and isoquercitrin. The 246th American Chemical Society (ACS) National Meeting. Indianapolis, IN, USA (September 8-12, 2013).

•    Cardioprotective and neuroprotective properties of apple flavonoids. University of Moncton, Moncton, NB (November 15, 2012).

•    Nutraceuticals from processing waste: Disease fighting properties of fruit bioactives. Atlantic Canada Bio-refinery Conference. Shediac, NB (June 19, 2012).

•    Antioxidant, nuroprotective and cardioprotective properties of apple flavonoids. The 50th National Conference of the Canadian Institute of Food science and Technology (CIFST), Niagara Falls, ON. (May 27, 2012).

•    Value-added fruit processing and human health: Global perspectives. International Conference on Postharvest Management and Technology for food Security, Jimma University, Jimma, Ethiopia (May 21, 2012).

•    Biological active compounds of cool climate fruits. Faculty of Natural Sciences, University of Addis Ababa, Addis Ababa, Ethiopia. (May 18, 2012).

•    Inhibition of angiotensin converting enzyme (ACE) by fruit flavonoids in regulation of hypertension. The second World Congress on Diabetes and Metabolism. Philadelphia, PN, USA (December 6-8, 2011).

•    Antioxidant properties of glycosylated flavonols. BIT’s 4th Annual World Congress of Industrial Biotechnology – 2011 (iBio2011),  Dalian, China (April 25-30, 2011).

•    Antioxidant capacity and PUFA oxidation inhibition properties of berry extracts. The 6th International Chemical Congress of Pacific Basin Societies (PacifiChem2010), Honolulu, Hawaii. USA. (December 15-20, 2010).

•    Value-added processing and bioproducts of fruit crops. The Southern Labrador Development Corporation, Labrador, NL. (July 29, 2010).

•    Antioxidant protection of omega-3 fatty acids by bioactive apple flavonoids. The 7th Annual Conference of Natural Health Product Research Society of Canada (NHPRS), Halifax, NS Pharmaceutical Biology 48S1: 16-17. (May 23 – 26, 2010).

•    Apple-derived antioxidants and value-added food products. The 146th Annual Meeting of the Nova Scotia Fruit Growers’ Association. Kentville, NS. (Jan. 28, 2010).

•    Flavonoids from fruits for human health promotion. Department of Pharmacology, Dalhousie University, Halifax, NS. (April 23, 2009)

•    Dietary quercetin glycosides: A review of current understanding of metabolism and antioxidant action. International Conference and Exhibition on Nutraceuticals and Functional Foods - 2008, Taichung, Taiwan. (November 14-17, 2008)